PacBio HiFi whole genome sequencing of adult male Anastrepha ludens W... - SRA

SRX25519850: PacBio HiFi whole genome sequencing of adult male Anastrepha ludens Willacy strain
1 PACBIO_SMRT (Sequel IIe) run: 3.8M spots, 34.6G bases, 18.2Gb downloads

Design: High molecular weight (HMW) DNA extraction was performed from the using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using theDiagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in lengthto generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a30hour movie time onone SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff.

Submitted by: United States Department of Agriculture

Study: Genome sequencing of Anastrepha ludens adult male: principal pseudohaplotype

PRJNA803324 • SRP358222 • All experiments • All runs

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Long-reads were used to generate a whole genome assembly of Anastrepha ludens, the Mexican fruit fly

Sample: Anastrepha ludens, Mexican fruit fly

SAMN25644084 • SRS11875074 • All experiments • All runs

Organism: Anastrepha ludens

Library:

Name: idAnaLude1_male_PacBio_CCS_low

Instrument: Sequel IIe

Strategy: WGS